Genetic models and the study of anaesthetic action
V.J. Simpson. University of Colorado Health Sciences Center, Department of Anaesthesiology, Children's Hospital, Denver, USA

Genetic model systems provide a novel approach to understanding mechanisms of anaesthetic drug action. Many models have been derived using selection processes that emphasise differential drug sensitivity, producing animal lines that differ in their CNS drug response. Studies of vertebrate (rodent) and invertebrate (Drosophila, Caenorhabditis elegans) animal model systems will be discussed. Lines can be derived from spontaneous and induced mutagenic processes, selectively bred lines, inbred lines possessing inherent differential drug sensitivities and targeted gene knockouts. The primary focus of the review is the general anaesthetic drugs that are commonly used in the clinical setting. These agents include the inhalational agents (halothane, enfurane, isoflurane and nitrous oxide) and the intravenous induction agents (propofol and diazepam). Rodent lines with differential sensitivity to opiates will also be included. Finally, an approach to isolating and identifying the genes that control anaesthetic sensitivity will be discussed in a section on mapping quantitative trait loci (QTL) in recombinant inbred lines